Faster protocols, consistent data.
Whether you´re a “Wild thing” or “Gewohnheitstier”, REAfinity™ Recombinant Antibodies will satisfy your need for faster protocols and consistent data. Discover the next-generation of flow antibodies and get inspired!
Low background signal – high reproducibility
All REAfinity™ Antibodies contain a specifically mutated human IgG1 Fc region that abolishes their binding to Fcγ receptors. This allows for background-free analysis and eliminates the need for additional blocking steps such as using a FcR blocking reagent.
view detailEliminate background without FcR blocking
Human PBMCs were stained with either a mouse monoclonal CD158a-PE antibody or REAfinity CD158a-PE (clone:REA284). Staining was performed without (top) and with (bottom) FcR block. The mouse monoclonal antibody binds unspecifically to CD56-negative cells (A). Staining with the REAfinity Antibody, shows no background signal. Cells were analyzed by flow cytometry on the MACSQuant® Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.
No FcR blocking step – the shortcut to your staining protocol
Staining protocols for immunophenotyping of human and mouse surface markers can be significantly faster with REAfinity Antibodies.
Traditional hybridoma clones
| Steps: | Time: |
|---|---|
| Prepare 106 cells | |
| Add blocking reagent to cells and incubate at 4°C or RT | 10–15 min. |
| Add primary antibodies and incubate at 4°C or RT | 10–30 min. |
| Wash cells | 1–2 x 10 min. |
| Resuspend and analyze cells | |
| Total staining procedure | 30–65 min. |
REAfinity Clones
| Steps: | Time: |
|---|---|
| Prepare 106 cells | |
| Add blocking reagent to cells and incubate at 4°C or RT | – |
| Add primary antibodies and incubate at 4°C or RT | 10 min. |
| Wash cells | 10 min. |
| Resuspend and analyze cells | |
| Total staining procedure | 20 min. |
