Faster protocols, consistent data.

Whether you´re a “Wild thing” or “Gewohnheitstier”, REAfinity™ Recombinant Antibodies will satisfy your need for faster protocols and consistent data. Discover the next-generation of flow antibodies and get inspired!

Low background signal – high reproducibility

All REAfinity™ Antibodies contain a specifically mutated human IgG1 Fc region that abolishes their binding to Fcγ receptors. This allows for background-free analysis and eliminates the need for additional blocking steps such as using a FcR blocking reagent.

Eliminate background without FcR blocking


Human PBMCs were stained with either a mouse monoclonal CD158a-PE antibody or REAfinity CD158a-PE (clone:REA284). Staining was performed without (top) and with (bottom) FcR block. The mouse monoclonal antibody binds unspecifically to CD56-negative cells (A). Staining with the REAfinity Antibody, shows no background signal. Cells were analyzed by flow cytometry on the MACSQuant® Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

No FcR blocking step – the shortcut to your staining protocol

Staining protocols for immunophenotyping of human and mouse surface markers can be significantly faster with REAfinity Antibodies.

Traditional hybridoma clones
Steps: Time:
Prepare 106 cells
Add blocking reagent to cells and incubate at 4°C or RT 10–15 min.
Add primary antibodies and incubate at 4°C or RT 10–30 min.
Wash cells 1–2 x 10 min.
Resuspend and analyze cells
Total staining procedure 30–65 min.
REAfinity Clones
Steps: Time:
Prepare 106 cells
Add blocking reagent to cells and incubate at 4°C or RT
Add primary antibodies and incubate at 4°C or RT 10 min.
Wash cells 10 min.
Resuspend and analyze cells
Total staining procedure 20 min.